What is Green Biotechnology? Uses, examples and benefits

Biotechnology is generally defined as the application of living organisms to create or modify products or processes for practical purposes. Within this multidisciplinary field, different types/colors are used to group its branches according to their application sectors. In this blog, we will focus on green biotechnology, explaining what it is, its applications, how it relates to environmental biotechnology, how it differs from other types, its benefits and risks, some practical examples, and finally, a review of the various colors of biotechnology and what each one represents.

What is green biotechnology?

Green biotechnology is the branch of biotechnology focused on agricultural and environmental fields. It includes the application of biotechnological techniques to plants, crops, and terrestrial ecosystems. In other words, it encompasses all biotechnological processes applied to the agricultural sector, including genetic improvement of plants, the use of beneficial microorganisms in agriculture, and the development of biological products for the field. Given its close relationship with plant sciences, it is sometimes also referred to as plant biotechnology or agro-biotechnology. Currently, many farmers around the world use these tools to combat pests, nourish crops, and make plants more resistant to diseases and extreme weather conditions (droughts, frosts, etc.).

What are the applications of green biotechnology?

Green biotechnology has a wide range of applications today. Some of the main ones include:

  • Transgenic resistant crops: Through recombinant DNA techniques, genetically modified plants resistant to pests, diseases, or herbicides have been developed. These crops (such as transgenic corn, soybeans, or cotton) have higher yields and require fewer chemical pesticides, reducing the use of agrochemicals in the field. For example, transgenic corn tolerant to glyphosate has been created, allowing farmers to control weeds without damaging the crop and reducing the amount of herbicide needed.
  • Biofertilizers and biopesticides: Another application is the development of biological inputs as alternatives to chemical fertilizers and pesticides. Biofertilizers are microorganisms (such as nitrogen-fixing bacteria or mycorrhizae) that, when inoculated into seeds or soils, increase nutrient availability for plants, naturally improving soil fertility. This reduces the use of chemical fertilizers while maintaining or even enhancing long-term soil quality. Similarly, biopesticides help control insects or fungi in a targeted way, reducing the need for synthetic pesticides and their environmental impacts.
  • Food and nutrition improvement: Green biotechnology also aims to obtain more nutritious and higher-quality foods. A prominent case is Golden Rice, a genetically modified rice variety that produces beta-carotene (provitamin A) in the grain, intended to combat vitamin A deficiency in populations where rice is a staple food. Additionally, oilseed crops have been modified to produce healthier vegetable oils (with less saturated fat), fruits enriched with vitamins, or crops with reduced toxin or allergen content. These improvements contribute to food security by providing healthier and more abundant food.
  • Biofuels and biodegradable materials: Another major application is the production of renewable energy and sustainable materials from biomass. For example, fermenting sugars from corn or sugarcane to obtain bioethanol, or using vegetable oils (such as rapeseed or palm) to produce biodiesel, are biotechnological processes that create biofuels, reducing dependence on fossil fuels. Research is also underway into obtaining biodegradable bioplastics from corn starch or other plant-based sources. These developments, sometimes part of what is called industrial white biotechnology, are closely linked to green biotechnology when the raw material comes from plant cultivation.

Green biotechnology can also contribute to environmental restoration. For example, through phytoremediation (using plants to absorb or degrade pollutants), it is possible to decontaminate soils with heavy metals or wastewater naturally. Biotechnological processes are also used in managing organic waste, such as producing compost from agricultural residues or anaerobic digestion to generate biogas. In summary, green biotechnology applications range from achieving more efficient and ecological agriculture to producing clean energy and helping to remediate the environment.

Infographic showing key applications of green biotechnology: transgenic crops, biofertilizers, improved nutrition, and renewable plant-based materials.

How is it related to environmental biotechnology?

Given its focus on sustainability, green biotechnology is closely linked to what is often called environmental biotechnology. Environmental biotechnology refers to the use of microorganisms, plants, or other living beings to protect, conserve, or restore the environment. It includes techniques such as bioremediation to clean up contaminated soils, treat wastewater, control gas emissions, or recycle waste—leveraging the ability of certain organisms to degrade toxic substances. This ecosystem-focused branch is typically classified under the color grey in biotechnology, as its main goal is conserving the natural environment through biological solutions.

Green and environmental biotechnologies share the goal of achieving a more harmonious interaction between technology and nature. In fact, many “green” applications have clear environmental benefits. For example, transgenic crops resistant to insects reduce the amount of chemical insecticides released into the environment, and biofertilizers lower soil and groundwater pollution by replacing synthetic fertilizers. Some genetically modified plants can even be directly used in bioremediation (absorbing heavy metals from soil, degrading explosives, etc.), blurring the line between green and grey biotechnologies.

In short, green biotechnology includes an important environmental aspect (making agriculture more sustainable and eco-friendlier), while environmental biotechnology (grey) specializes exclusively in addressing pollution and ecological conservation problems. They are complementary fields: green biotechnology creates cleaner, more efficient farming systems, and environmental biotechnology focuses on repairing damage and maintaining healthy ecosystems.

What are the benefits and risks of green biotechnology?

As we have seen, green biotechnology has the potential to make food and resource production more sustainable, but it also presents some challenges. Below are its main benefits and associated risks or concerns:

Benefits of green biotechnology:

  • Healthier and more sustainable agriculture: It enables the development of more nutritious crops (e.g., higher vitamin content) that are also safer, free from natural toxins or allergens. By creating pest- and disease-resistant plants, the use of pesticides and chemicals in fields is reduced, lessening soil and water contamination. This results in healthier food for consumers and more environmentally friendly agricultural practices.
  • Increased production and food security: Genetic improvements can boost crop yields and enable cultivation under difficult conditions (poor soils, drought, salinity). This helps produce more food with less land, supporting efforts to combat hunger and poverty in vulnerable regions. For example, certain modified varieties allow farmland to produce more, particularly benefiting developing countries facing growing food demand.

Risks and challenges of green biotechnology:

  • Loss of biodiversity and ecological imbalances: Widespread use of a few transgenic crops could lead to genetic erosion (loss of crop variety diversity). There are also concerns that introducing modified organisms could alter ecological interactions, affecting the balance of surrounding natural ecosystems. For example, if a transgenic plant crossbreeds with wild relatives, it could transfer competitive advantages and become an unwanted invasive species.
  • Unpredictable effects on health and environment: Although all commercial biotech crops undergo safety assessments, there is always a risk of unforeseen effects. Concerns have been raised about transgenic foods potentially causing new allergies in some people, or genetically modified organisms escaping from labs and disrupting natural communities. While there is no conclusive evidence of harm to human health from consuming approved GMOs, public perception of risk remains in some sectors.
  • Socioeconomic impact: The adoption of biotech technologies in agriculture may have economic and social consequences. On one hand, increased yields and automation could reduce the need for agricultural labor, affecting rural employment. On the other hand, many transgenic seeds are developed and patented by large companies, raising concerns about farmers’ dependence on these corporations and the possible exclusion of small producers unable to afford the new technologies. These issues call for regulatory frameworks and public policies to ensure that biotechnology benefits society fairly.

In summary, green biotechnology offers clear advantages, such as more productive, cleaner, and more nutritious agriculture, but also raises legitimate concerns regarding biodiversity preservation, ecological safety, public acceptance of transgenic foods, and economic fairness. A responsible approach involves evaluating these benefits and risks case by case, based on scientific evidence and solid regulations.

What are some examples of applied green biotechnology?

There are numerous examples showing how green biotechnology is currently applied in modern agriculture and beyond:

  • Large-scale transgenic crops: Several countries have adopted genetically modified crops. For example, in Argentina, nearly 100% of cultivated soybeans are transgenic (herbicide-tolerant), and more than 99% of planted corn is also transgenic, often combining insect resistance and herbicide tolerance. Similarly, nearly 100% of Argentine cotton contains Bt genes (insect resistance) plus herbicide tolerance. This high adoption rate reflects farmers’ confidence in the benefits of these crops, such as reduced insecticide use, higher yields, and lower production costs.

    Worldwide, countries like the United States, Brazil, India, China, Canada, and Paraguay grow millions of hectares of transgenic soy, corn, cotton, canola, and more. A notable example is Bt cotton, modified with a Bacillus thuringiensis gene to make it toxic to key pests; its introduction drastically reduced the amount of insecticides used in cotton fields, benefiting both farmers’ economics and the environment.

  • Nutritional and quality improvements: As mentioned, Golden Rice is a well-known case of green biotechnology aimed at fighting vitamin A deficiency. Although its commercial adoption has been slow, it represents a milestone in using transgenic crops for humanitarian purposes. Other examples include soybeans modified to produce healthier oil (higher omega-3 fatty acids, for instance), or biofortified corn with higher levels of essential amino acids for improved animal feed. Transgenic papaya varieties resistant to the “ringspot virus” have also been developed, saving the crop from pests that devastated its production (a successful case implemented in Hawaii since the 1990s). These illustrate how genetic engineering can solve specific agricultural problems and add nutritional or commercial value to crops.
  • Biofertilizers and organic agriculture: In various countries, biofertilizers are commercially available for different crops. For instance, specific Rhizobium strains are used to inoculate legume seeds (soy, beans, alfalfa), enabling them to fix atmospheric nitrogen in the soil and meet most of their nitrogen needs. Likewise, dried formulations of mycorrhizal fungi are applied in horticultural or forestry crops to enhance nutrient and water uptake by roots. These products, derived from biotechnology, allow for more “organic” agriculture by reducing reliance on synthetic chemicals. Regarding biopesticides, a common example is using Bacillus thuringiensis (or its purified toxins) to control insect larvae in crops like corn or vegetables, avoiding traditional insecticides. Specific viruses (entomopathogenic viruses) are also used as targeted bioinsecticides. These strategies have been made possible by biotechnological research that identified and multiplied such biological control agents.
  • Phytoremediation and environmental conservation: Green biotechnology is applied in plant-based bioremediation projects. An experimental example is the use of sunflowers and other plant species to extract heavy metals from contaminated soils near old mines or factories (the metals concentrate in plant tissues, which are then safely harvested and disposed of). Another case is genetically modified poplar trees that degrade industrial solvents in groundwater, cleaning up polluted aquifers.

    Additionally, in arid ecosystems, highly resistant plants (cacti, dry shrubs) supported by microbial biostimulants are being introduced to restore degraded lands. This relates to so-called brown biotechnology, a branch of green biotechnology adapted to desert areas. Although many of these examples are in research or pilot stages, they demonstrate green biotechnology’s versatility beyond food crops.

  • Fermented food production: Traditional biotechnological techniques have been applied in food production for centuries. The making of wine, beer, bread, yogurt, cheese, and other fermented products relies on microorganisms (yeasts, bacteria) that transform basic ingredients into goods with desired characteristics. While these processes have been known since antiquity, modern biotechnology has optimized many of these fermentations. For example, yeast strains have been selected for higher efficiency or specific flavors, and purified microbial enzymes are used in the dairy industry to improve cheese ripening. All of this is part of yellow biotechnology, showing that the line between “traditional” and “modern” is sometimes blurred: an artisanal process like fermentation can be enhanced thanks to advanced biotechnological knowledge.

What are the different colors of biotechnology and what do they represent?

In total, there are more than ten defined “colors” in biotechnology. The main ones (red, green, blue, white, yellow) were created to distinguish major areas of application, while others (grey, gold, brown, purple, orange, black) have been added for more specific fields as biotechnology continues to expand. This classification offers a broad overview of biotechnology’s potential across various sectors of society. Whether improving health, feeding us, caring for the planet, boosting industry, or exploring oceans and genetic data, biotechnology, in all its colors, plays a crucial role in the modern world and promises to be a key tool for building a more sustainable and innovative future. If you’re curious about the rest of the biotech colors, we’ve published a full blog that explores each one in depth.

What is green biotechnology?

Frequently Asked Questions (FAQ)

1. What is green biotechnology?

Green biotechnology applies biological techniques to agriculture and the environment, aiming to improve crops, reduce chemicals, and support sustainability.

2. What is green biotechnology used for?

It is used to develop pest-resistant crops, create biofertilizers and biopesticides, enhance nutritional value, and produce biofuels and biodegradable materials.

3. Is green biotechnology related to environmental biotechnology?

Yes. Green biotechnology overlaps with environmental biotech when applied to soil recovery, phytoremediation, or sustainable farming, though environmental biotech focuses more on pollution control.

4. What are examples of green biotechnology?

Examples include Golden Rice, Bt cotton, nitrogen-fixing biofertilizers, biodegradable plastics from corn, and phytoremediation with sunflowers.

5. What are the benefits of green biotechnology?

It improves yield, reduces chemical use, enhances food nutrition, and promotes eco-friendly farming.

6. What is the meaning of color in biotechnology?

Colors represent application areas: red (health), green (agriculture), blue (marine), white (industrial), yellow (food), grey (environmental), etc.

7. What is an example of environment biotechnology?

Using bacteria to clean up oil spills or plants to remove heavy metals from contaminated soil (phytoremediation) are common examples of environmental biotechnology.

References

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Cassette

We understand the importance of flexibility and efficiency in laboratory processes. That's why our equipment is designed to be compatible with Cassette filters, an advanced solution for a variety of filtration applications. Although we do not manufacture the filters directly, our systems are optimized to take full advantage of the benefits that Cassette filters offer.

Cassette filters are known for their high filtration capacity and efficiency in separation, making them ideal for ultrafiltration, microfiltration, and nanofiltration applications. By integrating these filters into our equipment, we facilitate faster and more effective processes, ensuring high-quality results.

Our equipment, being compatible with Cassette filters, offers greater versatility and adaptability. This means you can choose the filter that best suits your specific needs, ensuring that each experiment or production process is carried out with maximum efficiency and precision.

Moreover, our equipment stands out for its 100% automation capabilities. Utilizing advanced proportional valves, we ensure precise control over differential pressure, transmembrane pressure, and flow rate. This automation not only enhances the efficiency and accuracy of the filtration process but also significantly reduces manual intervention, making our systems highly reliable and user-friendly.

Hollow Fiber

We recognize the crucial role of flexibility and efficiency in laboratory processes. That's why our equipment is meticulously designed to be compatible with Hollow Fiber filters, providing an advanced solution for a broad spectrum of filtration applications. While we don't directly manufacture these filters, our systems are finely tuned to harness the full potential of Hollow Fiber filters.

Hollow Fiber filters are renowned for their exceptional performance in terms of filtration efficiency and capacity. They are particularly effective for applications requiring gentle handling of samples, such as in cell culture and sensitive biomolecular processes. By integrating these filters with our equipment, we enable more efficient, faster, and higher-quality filtration processes.

What sets our equipment apart is its 100% automation capability. Through the use of sophisticated proportional valves, our systems achieve meticulous control over differential pressure, transmembrane pressure, and flow rate. This level of automation not only boosts the efficiency and precision of the filtration process but also significantly diminishes the need for manual oversight, rendering our systems exceptionally reliable and user-friendly.

Contact General

Cellular configuration

The cellular configuration of the eLab Advanced is equipped with a pitched-blade impeller designed to support efficient mixing for cell culture processes in both laboratory development and early scale-up. The blade geometry promotes mainly axial flow, helping to distribute gases, nutrients and pH control agents uniformly throughout the vessel while keeping shear stress at a moderate level. This makes it suitable for mammalian, insect and other shear-sensitive cell lines when operated with appropriate agitation and aeration settings. In combination with the vessel aspect ratio and baffle design, the pitched blade supports stable foaming behavior and reproducible oxygen transfer, which is essential when comparing batches or transferring processes between working volumes.

Operators can fine-tune agitation speed to balance oxygen demand and mixing time without excessively increasing mechanical stress on the culture. 

Cellular configuration

The cellular configuration of the eLab Advanced is equipped with a pitched-blade impeller designed to support efficient mixing for cell culture processes in both laboratory development and early scale-up. The blade geometry promotes mainly axial flow, helping to distribute gases, nutrients and pH control agents uniformly throughout the vessel while keeping shear stress at a moderate level. This makes it suitable for mammalian, insect and other shear-sensitive cell lines when operated with appropriate agitation and aeration settings. In combination with the vessel aspect ratio and baffle design, the pitched blade supports stable foaming behavior and reproducible oxygen transfer, which is essential when comparing batches or transferring processes between working volumes.

Operators can fine-tune agitation speed to balance oxygen demand and mixing time without excessively increasing mechanical stress on the culture. 

Cellular configuration

The cellular configuration of the eLab Advanced is equipped with a pitched-blade impeller designed to support efficient mixing for cell culture processes in both laboratory development and early scale-up. The blade geometry promotes mainly axial flow, helping to distribute gases, nutrients and pH control agents uniformly throughout the vessel while keeping shear stress at a moderate level. This makes it suitable for mammalian, insect and other shear-sensitive cell lines when operated with appropriate agitation and aeration settings. In combination with the vessel aspect ratio and baffle design, the pitched blade supports stable foaming behavior and reproducible oxygen transfer, which is essential when comparing batches or transferring processes between working volumes.

Operators can fine-tune agitation speed to balance oxygen demand and mixing time without excessively increasing mechanical stress on the culture. 

Microbial configuration

The microbial configuration of the eLab Advanced is equipped with a Rushton turbine specifically designed for high-oxygen-demand processes such as bacterial and yeast fermentations. The radial-flow impeller generates strong mixing and intense gas dispersion, promoting high oxygen transfer rates and fast homogenization of nutrients, antifoam and pH control agents throughout the vessel. This makes it particularly suitable for robust microbial strains operating at elevated agitation speeds and aeration rates.

Operators can adjust agitation and gas flow to reach the required kLa while maintaining consistent mixing times, even at high cell densities. This configuration is an excellent option for users who need a powerful, reliable platform to develop and optimize microbial processes before transferring them to pilot or production scales.

Cellular configuration

The cellular configuration of the eLab Advanced is equipped with a pitched-blade impeller designed to support efficient mixing for cell culture processes in both laboratory development and early scale-up. The blade geometry promotes mainly axial flow, helping to distribute gases, nutrients and pH control agents uniformly throughout the vessel while keeping shear stress at a moderate level. This makes it suitable for mammalian, insect and other shear-sensitive cell lines when operated with appropriate agitation and aeration settings. In combination with the vessel aspect ratio and baffle design, the pitched blade supports stable foaming behavior and reproducible oxygen transfer, which is essential when comparing batches or transferring processes between working volumes.

Operators can fine-tune agitation speed to balance oxygen demand and mixing time without excessively increasing mechanical stress on the culture. 

Technical specifications

Materials and finishes

Typical
  • Product-contact parts: AISI 316L (1.4404), typical Ra < 0.4 µm (16 µin)
  • Non-contact parts/skid: AISI 304/304L
  • Seals/elastomers: platinum-cured silicone, EPDM and/or PTFE (material set depends on selection)
  • Elastomers compliance (depending on selected materials): FDA 21 CFR 177.2600 and USP Class VI
  • Surface treatments: degreasing, pickling and passivation (ASTM A380 and ASTM A968)
  • Roughness control on product-contact surfaces

Design conditions

Pressure & temperature

Defined considering non-hazardous process fluids (PED group 2) and jacket steam/superheated water (PED group 5), depending on configuration and project scope.

Reference design envelope
ModeElementWorking pressure (bar[g])Working pressure (psi[g])T max (°C / °F)
ProcessVessel0 / +2.50 / +36.3+90 / 194
ProcessJacket0 / +3.80 / +55.1+90 / 194
SterilisationVessel0 / +2.50 / +36.3+130 / 266
SterilisationJacket0 / +3.80 / +55.1+150 / 302
Jacket working pressure may also be specified as 0 / +4 bar(g) (0 / +58.0 psi[g]) depending on design selection; final values are confirmed per project.

Pressure control and safeguards

Typical
  • Designed to maintain a vessel pressure set-point typically in the range 0 to 2.5 bar(g)
  • Aseptic operation commonly around 0.2 to 0.5 bar(g) to keep the vessel slightly pressurised
  • Overpressure/underpressure safeguards included per configuration and regulations
  • Pressure safety device (e.g., rupture disc and/or safety valve) included according to configuration

Agitation

Reference ranges
Working volumeMU (Cell culture), referenceMB (Microbial), reference
10 L0 to 300 rpm0 to 1000 rpm
20 L0 to 250 rpm0 to 1000 rpm
30 L0 to 200 rpm0 to 1000 rpm
50 L0 to 180 rpm0 to 1000 rpm

Integrated peristaltic pumps (additions)

Typical

The equipment typically includes 4 integrated variable-speed peristaltic pumps for sterile additions (acid/base/antifoam/feeds). Actual flow depends on selected tubing and calibration.

ParameterTypical valueNotes
Quantity4 units (integrated)In control tower; assignment defined by configuration
Speed0-300 rpmVariable control from eSCADA
Minimum flow0-10 mL/minExample with 0.8 mm ID tubing; depends on tubing and calibration
Maximum flowUp to ~366 mL/minExample with 4.8 mm ID tubing; actual flow depends on calibration
Operating modesOFF / AUTO / MANUAL / PROFILEAUTO typically associated to pH/DO/foam loops or recipe
FunctionsPURGE, calibration, totaliser, PWMPWM available for low flow setpoints below minimum operating level

Gas flow control (microbial reference capacity)

Reference

For microbial culture (MB), gas flow controllers (MFC) are typically sized based on VVM targets. Typical reference VVM range: 0.5-1.5 (to be confirmed by process).

Working volume (L)VVM minVVM maxAir (L/min)O2 (10%) (L/min)CO2 (20%) (L/min)N2 (10%) (L/min)
100.51.55-150.5-1.51-30.5-1.5
200.51.510-301-32-61-3
300.51.515-451.5-4.53-91.5-4.5
500.51.525-752.5-7.55-152.5-7.5
O2/CO2/N2 values are shown as reference capacities for typical gas blending strategies (10% O2, 20% CO2, 10% N2). Final gas list and ranges depend on process and configuration.

Instrumentation and sensors

Typical

Instrumentation is configurable. The following list describes typical sensors integrated in standard configurations, plus common optional PAT sensors.

Variable / functionTypical technology / interfaceStatus (STD/OPT)
Temperature (process/jacket)Pt100 class A RTDSTD
Pressure (vessel/lines)Pressure transmitter (4-20 mA / digital)STD
Level (working volume)Adjustable probeSTD
pHDigital pH sensor (ARC or equivalent)STD
DO (pO2)Digital optical DO sensor (ARC or equivalent)STD
FoamConductive/capacitive foam sensorSTD
Weight / mass balanceLoad cell (integrated in skid)STD
pCO2Digital pCO2 sensor (ARC or equivalent)OPT
Biomass (permittivity)In-line or in-vessel sensorOPT
VCD / TCDIn-situ cell density sensorsOPT (MU)
Off-gas (O2/CO2)Gas analyser for OUR/CEROPT
ORP / RedoxDigital ORPOPT
Glucose / LactatePAT sensorOPT

Automation, software and connectivity

Typical

The platform incorporates TECNIC eSCADA (typically eSCADA Advanced for ePILOT) to operate actuators and control loops, execute recipes and manage process data.

Main software functions
  • Main overview screen with process parameters and trends
  • Alarm management (real-time alarms and historical log) with acknowledgement and comment option
  • Manual/automatic modes for actuators and control loops
  • Recipe management with phases and transitions; parameter profiles (multi-step) for pumps and setpoints
  • Data logging with configurable period and export to CSV; PDF report generation
Common control loops
  • Temperature control (jacket heating/cooling)
  • Pressure control (headspace) with associated valve management
  • pH control via acid/base addition pumps and optional CO2 strategy
  • DO control with cascade strategies (agitation, air, O2, N2) depending on package and configuration
  • Foam control (foam sensor and automatic antifoam addition)
Data integrity and 21 CFR Part 11

Support for 21 CFR Part 11 / EU GMP Annex 11 is configuration- and project-dependent and requires customer procedures and validation (CSV).

Utilities

Reference

Utilities depend on final configuration (e.g., AutoSIP vs External SIP) and destination market (EU vs North America). The following values are typical reference points.

UtilityTypical service / configurationPressureFlow / powerNotes
ElectricalEU base: 400 VAC / 50 Hz (3~)N/AAutoSIP: 12 kW; External SIP: 5 kWNA option: 480 VAC / 60 Hz; cabinet/wiring per NEC/NFPA 70; UL/CSA as required
Process gasesAir / O2 / CO2 / N2Up to 2.5 bar(g) (36.3 psi)According to setpointTypical OD10 pneumatic connections; final list depends on package
Instrument airPneumatic valvesUp to 6 bar(g) (87.0 psi)N/ADry/filtered air recommended
Cooling waterJacket cooling water2 bar(g) (29.0 psi)25 L/min (6.6 gpm)6-10 °C (43-50 °F) typical
Cooling waterCondenser cooling water2 bar(g) (29.0 psi)1 L/min (0.26 gpm)6-10 °C (43-50 °F) typical
Steam (External SIP)Industrial steam2-3 bar(g) (29.0-43.5 psi)30 kg/h (66 lb/h)For SIP sequences
Steam (External SIP)Clean steam1.5 bar(g) (21.8 psi)8 kg/h (18 lb/h)Depending on plant strategy

Compliance and deliverables

Typical

Depending on destination and project scope, the regulatory basis may include European Directives (CE) and/or North American codes. The exact list is confirmed per project and stated in the Declaration(s) of Conformity when applicable.

ScopeEU (typical references)North America (typical references)
Pressure equipmentPED 2014/68/EUASME BPVC Section VIII (where applicable)
Hygienic designHygienic design good practicesASME BPE (reference for bioprocessing)
Machine safetyMachinery: 2006/42/EC (until 13/01/2027) / (EU) 2023/1230OSHA expectations; NFPA 79 (industrial machinery) - project dependent
Electrical / EMCLVD 2014/35/EU; EMC 2014/30/EUNEC/NFPA 70; UL/CSA components and marking as required
Materials contactEC 1935/2004 + EC 2023/2006 (GMP for materials) where applicableFDA 21 CFR (e.g., 177.2600 for elastomers) - materials compliance
Software / CSVEU GMP Annex 11 (if applicable)21 CFR Part 11 (if applicable)
Standard documentation package
  • User manual and basic operating instructions
  • P&ID / layout drawings as per project scope
  • Material certificates and finish/treatment certificates (scope dependent)
  • FAT report (if included in contract)
Optional qualification and commissioning services
  • SAT (Site Acceptance Test)
  • IQ / OQ documentation and/or execution (scope agreed with customer)
  • CSV support package for regulated environments (ALCOA+ considerations, backups, time synchronisation, etc.)

Ordering and configuration

Project-based

ePILOT BR is configured per project. To define the right MU/MB package, volumes and options (utilities, sensors, software and compliance), please contact TECNIC with your URS or request the configuration questionnaire.

The information provided above is for general reference only and may be modified, updated or discontinued at any time without prior notice. Values and specifications are indicative and may vary depending on project scope, configuration and applicable requirements. This content does not constitute a binding offer, warranty, or contractual commitment. Any final specifications, deliverables and acceptance criteria will be confirmed in the corresponding quotation, technical documentation and/or contract documents.

Cellular configuration

The cellular configuration of the eLab Advanced is equipped with a pitched-blade impeller designed to support efficient mixing for cell culture processes in both laboratory development and early scale-up. The blade geometry promotes mainly axial flow, helping to distribute gases, nutrients and pH control agents uniformly throughout the vessel while keeping shear stress at a moderate level. This makes it suitable for mammalian, insect and other shear-sensitive cell lines when operated with appropriate agitation and aeration settings. In combination with the vessel aspect ratio and baffle design, the pitched blade supports stable foaming behavior and reproducible oxygen transfer, which is essential when comparing batches or transferring processes between working volumes.

Operators can fine-tune agitation speed to balance oxygen demand and mixing time without excessively increasing mechanical stress on the culture. 

Technical specifications

    ePILOT BR configuration questionnaire









    Project details



















    FAT:

    Shipment:

    Installation:

    SAT:

    IQ/OQ:


    Process and automation requirements























    MU only (cell culture)


    MB only (microbial)


    Utilities and infrastructure



    North America specific















    Connections, consumables and compliance












    EU specific




    North America specific


    Software / CSV (GMP)


    Validation, testing and documentation










    GMP / CSV


    Logistics and installation











    Additional comments




    Cellular configuration

    The cellular configuration of the eLab Advanced is equipped with a pitched-blade impeller designed to support efficient mixing for cell culture processes in both laboratory development and early scale-up. The blade geometry promotes mainly axial flow, helping to distribute gases, nutrients and pH control agents uniformly throughout the vessel while keeping shear stress at a moderate level. This makes it suitable for mammalian, insect and other shear-sensitive cell lines when operated with appropriate agitation and aeration settings. In combination with the vessel aspect ratio and baffle design, the pitched blade supports stable foaming behavior and reproducible oxygen transfer, which is essential when comparing batches or transferring processes between working volumes.

    Operators can fine-tune agitation speed to balance oxygen demand and mixing time without excessively increasing mechanical stress on the culture. 

    Technical specifications

    Models and working volumes

    Tank

    The ePlus Mixer platform combines an ePlus Mixer control tower with Tank frames and eBag 3D consumables. Tank can be supplied in square or cylindrical configurations (depending on project) to match the bag format.

    Tank modelNominal volumeMinimum volume to start agitation*
    Tank 50 L50 L15 L
    Tank 100 L100 L20 L
    Tank 200 L200 L30 L
    Tank 500 L500 L55 L
    *Values based on agitation start interlocks per tank model. Final performance depends on the selected eBag 3D, fluid properties and configuration.

    Design conditions and operating limits

    Reference

    Reference limits are defined for the ePlus Mixer and the Tank. It is recommended to validate the specific limits of the selected eBag 3D and single-use sensors for the customer’s process.

    ElementOperating pressureMaximum pressure (safety)Maximum working temperature
    ePlus Mixer (control tower)ATM0.5 bar(g)90 °C
    TankATM0.5 bar(g)45 °C
    Jacket (if applicable)N/A1.5 barDepends on utilities / scope
    The 0.5 bar(g) limit is associated with the equipment design, the circuit is protected by a safety valve. Confirm final limits on the equipment nameplate and project specification.

    Materials and finishes

    Typical
    • Control tower housing and frame: stainless steel 304
    • Product-contact metallic hard parts (if applicable): stainless steel 316 (defined in project manufacturing documentation)
    • Non-product-contact metallic parts: stainless steel 304
    • eBag consumable: single-use polymer (supplier dependent, gamma irradiation / sterilisation per specification)
    • Vent filters: PP (polypropylene), per component list
    For GMP projects, the recommended documentation package includes material certificates, surface finish certificates (Ra if applicable) and consumable sterility/irradiation certificates.

    Agitation system

    Magnetic

    Non-invasive magnetic agitation, the impeller is integrated in the eBag 3D Mixer format, avoiding mechanical seals. Agitation speed is controlled from the HMI, with start interlocks linked to the tank model and minimum volume.

    Reference speed range
    • Typical agitation range: 120 to 300 rpm (configuration dependent)
    • Magnetic drive motor (reference): Sterimixer SMA 85/140, 50 Hz, 230/400 V, 0.18 kW
    • Gear reduction (reference): 1:5
    • Actuation (reference): linear actuator LEYG25MA, stroke 30–300 mm, speed 18–500 mm/s (for positioning)
    Final rpm and mixing performance depend on tank size, bag format and process requirements.

    Weighing and volume control

    Integrated

    Weight and derived volume control are performed using 4 load cells integrated in the tank frame legs and a weight indicator. Tare functions are managed from the HMI to support preparation steps and additions by mass.

    ComponentReference modelKey parameters
    Load cells (x4)Mettler Toledo SWB505 (stainless steel)550 kg each, output 2 mV/V, IP66
    Weight indicatorMettler Toledo IND360 DINAcquisition and HMI display, tare and “clear last tare”
    For installation engineering, total floor load should consider product mass + equipment mass + margin (recommended ≥ 20%).

    Pumps and fluid handling

    Standard

    The platform includes integrated pumps for additions and circulation. Final tubing selection and calibration define the usable flow range.

    Included pumps (reference)
    • 3 integrated peristaltic pumps for additions (acid/base/media), with speed control from HMI
    • 1 integrated centrifugal pump for circulation / transfer (DN25)
    Peristaltic pumps (reference)
    ParameterReferenceNotes
    Quantity3 unitsIntegrated in the control tower
    Pump headHYB101 (Hygiaflex)Example tubing: ID 4.8 mm, wall 1.6 mm
    Max speed300 rpmSpeed control reference: 0–5 V
    Max flow (example)365.69 mL/minDepends on tubing and calibration
    Centrifugal pump (reference)
    ParameterReference
    ModelEBARA MR S DN25
    Power0.75 kW
    FlowUp to 42 L/min
    PressureUp to 1 bar
    For circulation and sensor loops, the eBag 3D format can include dedicated ports (depending on the selected consumable and application).

    Thermal management (optional jacket)

    Optional

    Tank can be supplied with a jacket (single or double jacket options). The thermal circuit includes control elements and a heat exchanger, enabling temperature conditioning depending on utilities and project scope.

    • Jacket maximum pressure (reference): 1.5 bar
    • Thermal circuit safety: pressure regulator and safety valve (reference set-point 0.5 bar(g))
    • Heat exchanger (reference): T5-BFG, 12 plates, alloy 316, 0.5 mm, NBRP
    • Solenoid valves (reference): SMC VXZ262LGK, 1", DC 24 V, 10.5 W
    • Jacket sequences: fill / empty / flush (scope dependent)
    The tank maximum temperature may depend on the thermal circuit and consumable limits. Confirm final values with the selected eBag 3D specification.

    Instrumentation and sensors

    Optional SU

    Single-use sensors can be integrated via dedicated modules. The following references describe typical sensors and interfaces listed in the datasheet.

    VariableReference modelInterface / protocolSupplyOperating temperatureIP
    pHOneFerm Arc pH VP 70 NTC (SU)Arc Module SU pH, Modbus RTU7–30 VDC5–50 °CIP67
    ConductivityConducell-P SU (SU)Arc Module Cond-P SU, Modbus RTU7–30 VDC0–60 °CIP64
    TemperaturePt100 ø4 × 52 mm, M8 (non-invasive)Analog / acquisition moduleProject dependentProject dependentProject dependent
    Measurement ranges and final sensor list depend on the selected single-use components and project scope.

    Automation, software and data

    Standard + options

    The ePlus SUM control tower integrates an industrial PLC and touch HMI. Standard operation supports Manual / Automatic / Profile modes, with optional recipe execution depending on selected software scope.

    Software scope (reference)
    • Standard: eBASIC (base HMI functions)
    • Optional: eSCADA Basic or eSCADA Advanced (project dependent)
    • Trends, alarms and profiles, profiles up to 100 steps (depending on scope)
    • Data retention (reference): up to 1 year
    Connectivity (reference)
    • Industrial Ethernet and integrated OPC server (included)
    • Remote access option (project dependent)

    Utilities and facility interfaces

    Typical

    Installation requirements depend on jacket and temperature scope and the customer layout. The following values are typical references.

    UtilityPressureFlowConnectionsNotes
    Electrical supplyN/AReference: 18 A380–400 VAC, 3~ + N, 50 HzConfirm per final configuration and destination market
    EthernetN/AN/ARJ45OPC server, LAN integration
    Tap water2.5 barN/A1/2" (hose connection)Jacket fill and services, tank volume about 25 L
    Cooling water2–4 bar10–20 L/min2 × 3/4" (hose connection)Heat exchanger and jacket cooling
    Process air2–4 barN/A1/2" quick couplingUsed for jacket emptying
    DrainN/AN/A2 × 3/4" (hose connection)For draining
    ExhaustN/AN/AN/AOptional (depending on project)
    Stack light (optional)N/AN/AN/A3-colour indication, as per scope
    During FAT, verify in the installation checklist that the available utilities match the selected configuration and scope.

    Documentation and deliverables

    Project-based

    Deliverables depend on scope and project requirements. The following items are typical references included in the technical documentation package.

    • Datasheet and user manual (HMI and system operation)
    • Electrical schematics, PLC program and backup package (scope dependent)
    • P&ID, layout and GA drawings (PDF and/or CAD formats, project dependent)
    • Factory Acceptance Test (FAT) protocol and FAT report (as per contract)
    • Installation checklist
    • Material and consumable certificates, as required for regulated projects (scope dependent)
    On-site services (SAT, IQ/OQ) and extended compliance packages are optional and defined per project.

    Ordering and configuration

    Contact

    The ePlus Mixer scope is defined per project. To select the right tank size, bag format, sensors and optional jacket and software, please share your URS or request the configuration questionnaire.

    The information provided above is for general reference only and may be modified, updated or discontinued at any time without prior notice. Values and specifications are indicative and may vary depending on project scope, configuration and applicable requirements. This content does not constitute a binding offer, warranty, or contractual commitment. Any final specifications, deliverables and acceptance criteria will be confirmed in the corresponding quotation, technical documentation and/or contract documents.